ELECTROPHORESIS OF LIPID-FREE BLOOD SERUM

Electrophoresis of Lipid-free Blood Serum

The finding of lipid substances in all electrophoretically separable serum proteins, in the case of w and ,&globulins in remarkably large proportions (Blix, Tiselius, and Svensson, 1941), raised the question of the possible r&e of the lipids in the electrophoretic behavior of these proteins. Experiments with sera from which the lipids had been largely removed, according to the mild extraction p...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Simplified determination of the lipid components of blood serum.

A simplified scheme of analysis is presented for the determination of free and esterified cholesterol, the phospholipids, and the neutral fats (triglycerides) of blood serum. The hydroxamic acid reaction is used to determine total esterified fatty acids. The phospholipids are determined after acid digestion of a trichloroacetic acid precipitate of protein-phospholipid, and both total and free c...

Serum Protein Electrophoresis

electrophoresis for detection of DNA point mutations. Journal of Chromatography B 697: 195}205. Righetti PG and GelR C (1998) Analysis of clinicallyrelevant, diagnostic DNA by capillary zone and double-gradient gel slab electrophoresis. Journal of Chromatography A 806: 97}112. Thormann W (1997) Drug monitoring by capillary electrophoresis. In Wong SHY, Sunshine I (Eds) Analytical Therapeutic Dr...

Free flow electrophoresis of chloroplasts.

Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis...